The lambda F mutants belong to two cistrons.

نویسندگان

  • C E Boklage
  • E C Wong
  • V C Bode
چکیده

Amber mutants previously mapped at seven sites in head gene F exhibit two contrasting phenotypes. Mutant F423, located in the right half of gene F, makes defective head particles with normal size lambdaDNA. These particles are complemented by crude lysates of mutants in other head genes. Mutants F785, F471 and F730 map in three adjacent sites in the left half of gene F. They are not complemented by lysates of other head mutants and do not produce headlike particles. Their lysates complement the incomplete heads present in F423. In vivo all three mutants are complemented by F423 but not by each other.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Temperature-sensitive mutants of bacteriophage mu.

Temperature-sensitive mutants of bacteriophage Mu, which grow at 32 C but not at 42 C, have been isolated. These mutants fall into two groups. Group 1 mutants fail to lyse host cells at nonpermissive temperatures, whereas lysis occurs normally with the group 2 mutants. All of the group 1 mutants apparently belong to the cistrons mapping to the left of gene C, whereas the group 2 mutants have le...

متن کامل

Genetic analysis of bacteriophage Mu-induced flagellar mutants in Escherichia coli.

In previous work, at least 10 discrete cistrons involved in the synthesis of flagella in Escherichia coli were described. Six cistrons were located between his and uvrC on the genetic map. These were referred to as hag, flaA, flaB, flaC, flaD, and flaE. Four cistrons referred to as mot, flaG, flaH, and flaI were located between uvrC and aroD. In order to determine whether these genes are organi...

متن کامل

Genetic analysis of transfer by the Escherichia coli sex factor F, using P1 transductional complementation.

P1 transduction has been used to perform a complementation analysis of a series of transfer-deficient mutants of Flac. The results define ten cistrons and are consistent with the results of a conjugational analysis presented in an accompanying report. Both sets of results are summarized here. Between them, they define eleven cistrons, traA through traK, necessary for conjugational deoxyribonucl...

متن کامل

Genetic analysis of flagellar mutants in Escherichia coli.

Flagellar mutants in Escherichia coli were obtained by selection for resistance to the flagellotropic phage chi. F elements covering various regions of the E. coli genome were then constructed, and, on the basis of the ability of these elements to restore flagellar function, the mutations were assigned to three regions of the E. coli chromosome. Region I is between trp and gal; region II is bet...

متن کامل

Isolation and characterization of flightless mutants in Drosophila melanogaster.

Since animal behaviour is executed through neuronal circuits including sensory receptors and muscle, genes vital for their development and differentiation must be found among mutants having behavioural anomaly. After mutagenesis with ethyl methanesulphonate (EMS), we screened for X-linked flightless mutants of Drosophila melanogaster by using column-type flight tester. Approximately 10(4) indiv...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Genetics

دوره 75 2  شماره 

صفحات  -

تاریخ انتشار 1973